Cloning Vectors

A biological research vector may be a little piece of polymer, taken from a pandemic, a plasmid, or the cell of a better organism, that may be stably maintained in an organism, and into that a distant polymer fragment is inserted for biological research functions. There square measure many varieties of biological research vectors; however the foremost ordinarily used ones square measure genetically designed plasmids. All biological research vectors have options that permit a cistron to be handily inserted into the vector or far away from it. A selectable marker is carried by the vector to permit the choice of completely reworked cells. A biological research vector needn't contain appropriate parts for the expression of a cloned target cistron, like a promoter and ribosomal binding web site (RBS); several but do, and should then work as AN expression vector. Plasmids square measure autonomously replicating circular extra-chromosomal polymer. The bacteriophages used for biological research square measure the virus λ and M13 virus.

  • Features of a cloning vector
  • Types of cloning vectors
  • Gateway Technology
  • Plant transformation vector
  • Golden Gate Cloning

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